Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZBP1 in samples. An antibody specific for ZBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Using fragments derived from these exons, EST database searching, and PCR analysis, they cloned human DLM1 cDNA. They also cloned a rat DLM1 cDNA. Human DLM1 encodes a deduced 429-amino acid protein with 47% and 46% sequence identity with the mouse and rat homologs, respectively. All 3 proteins contain 4 conserved regions, 2 of which are homologous to the Z-DNA binding domains Z-alpha and Z-beta of the RNA editing enzyme ADAR.
By RT-PCR of human tissues, Rothenburg et al. (2002) found prominent expression of DLM1 in the small intestine and lymphatic tissues, including lymph node, leukocytes and spleen; detectable expression in thymus, lung, liver, and pancreas; and no expression in brain and skeletal muscle